Difference between revisions of "Sandbox"

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* '''Description:''' transcriptional activator involved in the degradation of glutamine phosphoribosylpyrophosphate amidotransferase <br/><br/>
+
* '''Description:''' forespore-specific protein, controls SigG activation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''degA''
+
|''csfB''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaM ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator (LacI family)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || forespore-specific protein
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of SigG activation
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.784  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 7.671  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1011 bp, 337 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 192 bp, 64 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yisR]]'', ''[[yisS]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rrnA-5S]]'', ''[[xpaC]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12923&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11800&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:degA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:csfB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 +
premature activation of [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csfB.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10847]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10088]
  
 
=== Additional information===
 
=== Additional information===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' prevents premature activation of SigG [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37947 P37947]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37534 P37534]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU10840]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00240]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''csfB''
  
−
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigF]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
 +
# Wang et al. (2006) The forespore line of gene expression in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''358:''' 16-37. [http://www.ncbi.nlm.nih.gov/sites/entrez/16497325 PubMed]
 +
# Chary et al. (2007) Expression of the sigmaF-directed ''csfB'' locus prevents premature appearance of sigmaG activity during sporulation of ''Bacillus subtilis''.''J. Bacteriol.'' '''189:''' 8754-8757. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921305 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 10:13, 11 May 2009

  • Description: forespore-specific protein, controls SigG activation

Gene name csfB
Synonyms yaaM
Essential no
Product forespore-specific protein
Function control of SigG activation
MW, pI 7 kDa, 7.671
Gene length, protein length 192 bp, 64 aa
Immediate neighbours rrnA-5S, xpaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CsfB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

premature activation of SigG PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: prevents premature activation of SigG PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: csfB
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Wang et al. (2006) The forespore line of gene expression in Bacillus subtilis. J. Mol. Biol. 358: 16-37. PubMed
  2. Chary et al. (2007) Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis.J. Bacteriol. 189: 8754-8757. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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