Difference between revisions of "Sandbox"

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* '''Description:''' late competence protein required for DNA binding and uptake <br/><br/>
+
* '''Description:''' non-essential gene for competence <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''comEB''
+
|''comER''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''comD ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''comD, comED ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || late competence protein
+
|style="background:#ABCDEF;" align="center"| '''Product''' || putative pyrroline-5'-carboxylate reductase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic compentence
+
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic compentence  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.368  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 6.183  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 567 bp, 189 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 819 bp, 273 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[comEC]]'', ''[[comEA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[comEA]]'', ''[[yqeM]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14500&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14502&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:comEB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:comER_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comEABC.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comER.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10481]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10479]
  
 
=== Additional information===
 
=== Additional information===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P32393 P32393]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39696 P39696]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25580]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25600]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[comEA]]-[[comEB]]-[[comEC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7968523 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
+
* '''Operon:''' ''comER'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7968523 PubMed]
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:'''  
+
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] 
  
* '''Regulatory mechanism:''' post-translational control by [[ComN]], ''[[yutB]]'' is required for transcription [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
+
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 118: Line 118:
 
=References=
 
=References=
  
# Ogura, M. and T. Tanaka (2009) The ''Bacillus subtilis'' Late Competence Operon ''comE'' Is Transcriptionally Regulated by ''yutB'' and under Post-Transcription Initiation Control by ''comN'' (''yrzD'') ''J. Bacteriol.'' '''191:''' 949-958. [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
+
# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 10:13, 11 May 2009

  • Description: non-essential gene for competence

Gene name comER
Synonyms comD, comED
Essential no
Product putative pyrroline-5'-carboxylate reductase
Function genetic compentence
MW, pI 30 kDa, 6.183
Gene length, protein length 819 bp, 273 aa
Immediate neighbours comEA, yqeM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ComER context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed