Difference between revisions of "PurR"
| Line 76: | Line 76: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed] | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1P4A 1P4A], 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed] |
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37551 P37551] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37551 P37551] | ||
Revision as of 21:51, 5 May 2009
- Description: transcription repressor of the pur operon
| Gene name | purR |
| Synonyms | yabI |
| Essential | no |
| Product | transcription repressor of the pur operon |
| Function | regulation of purine biosynthesis |
| MW, pI | 31 kDa, 5.771 |
| Gene length, protein length | 855 bp, 285 aa |
| Immediate neighbours | ispE, yabJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Biological activity: transcriptional repressor of purine biosynthetic operons
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed
- Localization:
Database entries
- Swiss prot entry: P37551
- KEGG entry: [3]
- E.C. number:
Additional information
requires YabJ for in vivo activity
Expression and regulation
- Regulation: negative autoregulation, induction in the presence of PRPP PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Weng et al. (1995) Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92: 7455-7459. PubMed
- Weng & Zalkin (2000) Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.Currr. Microbiol. 41: 56-59. PubMed
- Sinha et al. (2003) The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.J. Bacteriol. 185: 4087-4098. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
