ComK

• Description: competence transcription factor (CTF)
  
  

Gene name	comK
Synonyms
Essential	no
Product	competence transcription factor (CTF)
Function	regulation of competence and DNA uptake
MW, pI	22 kDa, 7.669
Gene length, protein length	576 bp, 192 aa
Immediate neighbours	yhzC, yhxD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MecA-ComK

• Localization:

Database entries

• Structure:

• Swiss prot entry: P40396

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

References

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Smits, W.K., Bongiorni, C., Veening, J.W., Hamoen, L.W., Kuipers, O.P. & Perego, M. (2007) Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis. Mol. Microbiol. 65, 103-120. PubMed
3. Smits, W.K., Hoa, T.T., Hamoen, L.W., Kuipers, O.P. & Dubnau, D. (2007) Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein. Mol. Microbiol. 64, 368-381. PubMed
4. Smits, W.K., Kuipers, O.P. & Veening, J.W. (2006) Phenotypic variation in bacteria: the role of feedback regulation. Nat. Rev. Microbiol. 4, 259-271. PubMed
5. Susanna, K.A., den Hengst, C.D., Hamoen, L.W. & Kuipers, O.P. (2006) Expression of Transcription Activator ComK of Bacillus subtilis in the Heterologous Host Lactococcus lactis Leads to a Genome-Wide Repression Pattern: a Case Study of Horizontal Gene Transfer. Appl. Environ. Microbiol. 72, 404-411. PubMed
6. Susanna, K.A., Fusetti, F., Thunnissen, A.M., Hamoen, L.W. & Kuipers, O.P. (2006) Functional analysis of the competence transcription factor ComK of Bacillus subtilis by characterization of truncation variants. Microbiology 152, 473-483. PubMed
7. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed