Difference between revisions of "Sandbox"

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* '''Description:''' modulator of CtsR-dependent repression, McsA activates kinase activity of McsB <br/><br/>
+
* '''Description:''' small acid-soluble spore protein (minor) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mcsA''
+
|''sspF''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yacH ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || activator of McsB kinase activity
+
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (minor)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of CtsR activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 6.624  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 555 bp, 185 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 183 bp, 61 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ctsR]]'', ''[[mcsB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11860&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11821&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:mcsA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspF.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10146]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10108]
  
 
=== Additional information===
 
=== Additional information===
Line 70: Line 70:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[McsA]]-[[McsB]],  [[McsA]]-[[MscB]]-[[CtsR]],  [[McsA]]-[[McsB]],  [[McsA]]-[[MscB]]-[[CtsR]],  [[McsA]]-[[McsB]]
+
* '''Interactions:'''
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00840]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00450]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''sspF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]  
+
* '''[[Sigma factor]]:''' [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
+
* '''Regulation:''' switched on during stage III of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
+
# Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''176:''' 333-348. [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 22:47, 28 April 2009

  • Description: small acid-soluble spore protein (minor)

Gene name sspF
Synonyms
Essential no
Product small acid-soluble spore protein (minor)
Function protection of spore DNA
MW, pI 6 kDa, 9.647
Gene length, protein length 183 bp, 61 aa
Immediate neighbours veg, ispE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SspF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: switched on during stage III of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis. J. Mol. Biol. 176: 333-348. PubMed