Difference between revisions of "ClpE"
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− | * '''Mutant:''' | + | * '''Mutant:''' ''clpX::spec'' available from the [[Hamoen]] Lab [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Subtiwiki] |
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* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
− | * '''GFP fusion:''' | + | * '''GFP fusion:''' C-terminal YFP and CFP fusions (single copy) available from the [[Hamoen]] Lab [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Subtiwiki] |
* '''two-hybrid system:''' | * '''two-hybrid system:''' |
Revision as of 12:49, 28 April 2009
- Description: ATP-dependent Clp protease-like (class III stress gene)
Gene name | clpE |
Synonyms | ykvH |
Essential | no |
Product | ATP-dependent Clp protease-like |
Function | protein degradation |
MW, pI | 77 kDa, 5.135 |
Gene length, protein length | 2097 bp, 699 aa |
Immediate neighbours | motA, ykvI |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): ClpC
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: expressed after heat shock, localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed