Difference between revisions of "Sandbox"

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* '''Description:''' transcription repressor of class III heat shock genes (clpC operon, clpE, clpP) <br/><br/>
+
* '''Description:''' modulator of CtsR-dependent repression, McsA activates kinase activity of McsB <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ctsR''
+
|''mcsA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yacG ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yacH ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor
+
|style="background:#ABCDEF;" align="center"| '''Product''' || activator of McsB kinase activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of protein degradation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of CtsR activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.261  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 6.624  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 462 bp, 154 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 555 bp, 185 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rrnW-5S]]'', ''[[mcsA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ctsR]]'', ''[[mcsB]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11859&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11860&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ctsR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:mcsA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10145]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10146]
  
 
=== Additional information===
 
=== Additional information===
Line 68: Line 68:
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:''' probably activated by dephosphorylation by [[McsA]] and inactivated by phosphorylation by [[McsB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/14984053 PubMed]
+
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[McsA]]-[[MscB]]-[[CtsR]],  [[McsA]]-[[MscB]]-[[CtsR]]  
+
* '''Interactions:''' [[McsA]]-[[McsB]],  [[McsA]]-[[MscB]]-[[CtsR]],  [[McsA]]-[[McsB]],  [[McsA]]-[[MscB]]-[[CtsR]],  [[McsA]]-[[McsB]]
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00830]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00840]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 119: Line 119:
  
 
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
 
# Petersohn et al. (2001) Global Analysis of the General Stress Response of ''Bacillus subtilis''. ''J Bacteriol.'' '''183:''' 5617-5631 [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
# Krüger E, Hecker M. (1998) The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. ''J Bacteriol.'' '''180(24):''' 6681-8.. [http://www.ncbi.nlm.nih.gov/sites/entrez/9852015 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:46, 28 April 2009

  • Description: modulator of CtsR-dependent repression, McsA activates kinase activity of McsB

Gene name mcsA
Synonyms yacH
Essential no
Product activator of McsB kinase activity
Function control of CtsR activity
MW, pI 20 kDa, 6.624
Gene length, protein length 555 bp, 185 aa
Immediate neighbours ctsR, mcsB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
McsA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed