Difference between revisions of "ClpX"
(→Extended information on the protein) |
(→Biological materials) |
||
| Line 102: | Line 102: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' ''clpx::kan'' and ''clpX::spec'' available from the [[Hamoen]] Lab [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Subtiwiki] |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 108: | Line 108: | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| − | * '''GFP fusion:''' | + | * '''GFP fusion:''' C-terminal GFP fusions (both single copy and 2th copy in ''amyE'' locus, also as CFP and YFP variants) available from the [[Hamoen]] Lab [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Subtiwiki] |
* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
Revision as of 12:39, 28 April 2009
- Description: ATP-dependent Clp protease ATP-binding subunit (class III heat-shock protein)
| Gene name | clpX |
| Synonyms | |
| Essential | no |
| Product | ATP-dependent Clp protease ATP-binding subunit |
| Function | protein degradation |
| MW, pI | 46 kDa, 4.645 |
| Gene length, protein length | 1260 bp, 420 aa |
| Immediate neighbours | lonB, tig |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with ClpP
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion: C-terminal GFP fusions (both single copy and 2th copy in amyE locus, also as CFP and YFP variants) available from the Hamoen Lab Subtiwiki
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage
Your additional remarks
References
- Gerth, U., Krüger, E., Derré, I., Msadek, T. and Hecker, M. 1998. Stress induction of the Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of the ClpP protease and the involvement of ClpP and ClpX in stress tolerance. Mol. Microbiol. 28: 787-802. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
