Difference between revisions of "Sandbox"

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* '''Description:''' phosphodiesterase/alkaline phosphatase <br/><br/>
+
* '''Description:''' component of the TatAdCd twin-arginine translocase<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''phoD''
+
|''tatAD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycbS ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yczB ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphodiesterase/alkaline phosphatase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || component of the twin-arginine <br>translocation pathway
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || aquisition of phosphate upon phosphoate starvation 
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protein secretion
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 8.394  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 9.693  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1668 bp, 556 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 210 bp, 70 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycbR]]'', ''[[tatAD]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phoD]]'', ''[[tatCD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12056&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12057&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:phoD_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:tatAD_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 41: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoD.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11174]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12777]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' translocation of [[PhoD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 70: Line 72:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[TatAD]]-[[PhoD]],  
+
* '''Interactions:''' [[TatAD]]-[[PhoD]], [[TatAD]]-[[TatCD]], [[TatAD]]-[[TatCY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
  
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed], secreted by the [[TatAD]]-[[TatCD]] complex  [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
* '''Localization:''' forms foci at the division sites and cell poles, localization requires interaction with [[TatCD]] or [[TatCY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU02620]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU02630]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 121: Line 123:
 
=References=
 
=References=
  
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:48, 27 April 2009

  • Description: component of the TatAdCd twin-arginine translocase

Gene name tatAD
Synonyms yczB
Essential no
Product component of the twin-arginine
translocation pathway
Function protein secretion
MW, pI 7 kDa, 9.693
Gene length, protein length 210 bp, 70 aa
Immediate neighbours phoD, tatCD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TatAD context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: translocation of PhoD PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: forms foci at the division sites and cell poles, localization requires interaction with TatCD or TatCY PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed