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| − | * '''Description:''' xylose isomerase <br/><br/>
| + | #REDIRECT [[SkfE]] |
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''xylA''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || xylose isomerase
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of xylan and xylose
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.635
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1335 bp, 445 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[xylR]]'', ''[[xylB]]''
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13644]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:xylA_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
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| − | <br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Coordinates:'''
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| − | ===Phenotypes of a mutant ===
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/xylAB.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10806]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | * '''Protein family:'''
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| − | * '''Paralogous protein(s):'''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:'''
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| − | * '''Localization:'''
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| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''Swiss prot entry:'''
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU17600]
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| − | * '''E.C. number:''' 5.3.1.5
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| − | | |
| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[xylA]]-[[xylB]]''
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| − | * '''[[Sigma factor]]:''' [[SigA]]
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| − | * '''Regulation:''' repressed by glucose ([[CcpA]]) , carbon catabolite repression ([[CcpA]]), induction by xylose ([[XylR]])
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| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[CcpA]]: transcription repression, [[XylR]]: transcription repression
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:'''
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| − |
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:'''
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| − | =Labs working on this gene/protein=
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| − | [[Wolfgang Hillen]], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | # Dahl, M. K., J. Degenkolb, and W. Hillen. 1994. Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs. J. Mol. Biol. 243:413-424. [http://www.ncbi.nlm.nih.gov/sites/entrez/7966270 PubMed]
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| − | # Jacob S, Allmansberger R, Gärtner D, Hillen W (1991) Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame. Mol Gen Genet 229: 189-196. [http://www.ncbi.nlm.nih.gov/sites/entrez/1921970 PubMed]
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| − | # Kraus A, Hueck C, Gärtner D, Hillen W (1994) Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression. J Bacteriol 176: 1738-1745. [http://www.ncbi.nlm.nih.gov/sites/entrez/8132469 PubMed]
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| − | # Gärtner D, Geißendörfer M, Hillen W. 1988. Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose. J. Bacteriol. 170:3102-3109. [http://www.ncbi.nlm.nih.gov/sites/entrez/2454911 PubMed]
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| − | # Gärtner D, Degenkolb J, Ripperger JAE, Allmansberger R, Hillen W. 1992. Regulation of the Bacillus subtilis W23 xylose utilization operon: interaction of Xyl repressor with xyl operator and the inducer xylose. Mol. Gen. Genet. 232:415-422 [http://www.ncbi.nlm.nih.gov/sites/entrez/1588910 PubMed]
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| − | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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