Difference between revisions of "Sandbox"

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* '''Description:''' general stress protein, survival of ethanol  stress, spore coat protein <br/><br/>
  
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''yaaH''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || survival of ethanol  stress
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.618 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1281 bp, 427 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dgk]]'', ''[[yaaI]]''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11792&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:yaaH_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|}
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__TOC__
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<br/><br/>
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=The gene=
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=== Basic information ===
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* '''Coordinates:'''
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===Phenotypes of a mutant ===
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=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaH.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10080]
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=== Additional information===
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=The protein=
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=== Basic information/ Evolution ===
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* '''Catalyzed reaction/ biological activity:'''
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* '''Protein family:'''
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* '''Paralogous protein(s):'''
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=== Extended information on the protein ===
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* '''Kinetic information:'''
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* '''Domains:'''
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* '''Modification:'''
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* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
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* '''Interactions:'''
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* '''Localization:''' spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed]
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=== Database entries ===
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* '''Structure:'''
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* '''Swiss prot entry:'''
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00160]
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* '''E.C. number:'''
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=== Additional information===
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required for survival of ethanol stress [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
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=Expression and regulation=
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* '''Operon:''' ''yaaH'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
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* '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
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* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed], expressed during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
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* '''Regulatory mechanism:'''
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* '''Additional information:'''
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=Biological materials =
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* '''Mutant:'''
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* '''Expression vector:'''
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
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* '''two-hybrid system:'''
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* '''Antibody:'''
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=Labs working on this gene/protein=
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=Your additional remarks=
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=References=
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# H&ouml;per et al. (2005) Comprehensive Characterization of the Contribution of Individual [[SigB]]-Dependent General Stress Genes to Stress Resistance of ''Bacillus subtilis''. J. Bact. '''187:''' 2810-2826  [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
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# Kodama et al. (1999) The ''Bacillus subtilis yaaH'' gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.''J. Bacteriol.'' '''181:''' 4584-4591. [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
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# Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of ''Bacillus subtilis''.''J.Bacteriol.'' '''187:''' 2810-2826. [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
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# Aronson (2002) Sporulation and delta-endotoxin synthesis by ''Bacillus thuringiensis''.''Cell. Mol. Life Sci.'' '''59:''' 417-425. [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed]

Revision as of 22:09, 21 April 2009

  • Description: general stress protein, survival of ethanol stress, spore coat protein

Gene name yaaH
Synonyms
Essential no
Product spore coat protein
Function survival of ethanol stress
MW, pI 48 kDa, 5.618
Gene length, protein length 1281 bp, 427 aa
Immediate neighbours dgk, yaaI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YaaH context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: spore coat PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

required for survival of ethanol stress PubMed

Expression and regulation

  • Regulation: induced by stress (SigB) PubMed, expressed during sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis. J. Bact. 187: 2810-2826 PubMed
  2. Kodama et al. (1999) The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.J. Bacteriol. 181: 4584-4591. PubMed
  3. Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.J.Bacteriol. 187: 2810-2826. PubMed
  4. Aronson (2002) Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.Cell. Mol. Life Sci. 59: 417-425. PubMed
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