Difference between revisions of "Sandbox"

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* '''Description:''' trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC <br/><br/>
+
* '''Description:''' cell wall-associated protein precursor <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglP''
+
|''wapA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBC
+
|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall-associated protein precursor
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, control of LicT activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 258 kDa, 6.131  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 7002 bp, 2334 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxxG]]'', ''[[yxxF]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15963&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15959&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:bglP_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:wapA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 43: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/wapA-yxxG.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10797]
  
 
=== Additional information===
 
=== Additional information===
Line 56: Line 56:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' PTS permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 74: Line 74:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:'''
+
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 82: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39270]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39230]
  
* '''E.C. number:''' 2.7.1.69
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
+
* '''Operon:''' ''[[wapA]]-[[yxxG]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' repressed by glucose ([[CcpA]]) ,  induced by salicin  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed], repressed by glucose (catabolite repression)
+
* '''Regulation:'''  
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression, Induction: [[LicT]]-dependent RNA switch (antitermination), catabolite repression: repression by [[CcpA]]  ([[CcpA]] binding site overlaps -35 region) and lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
+
* '''Regulatory mechanism:''' activated by [[YvrHb]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 120: Line 120:
 
=References=
 
=References=
  
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''. ''Microbiology'' '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
+
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 
+
# Serizawa M, Kodama K, Yamamoto H, Kobayashi K, Ogasawara N, Sekiguchi J. (2005) Functional analysis of the YvrGHb two-component system of ''Bacillus subtilis'': identification of the regulated genes by DNA microarray and northern blot analyses. ''Biosci. Biotechnol. Biochem.'' '''69:''' 2155-2169. [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
# Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in ''Bacillus subtilis'': The ''bglP'' gene product has both transport and regulatory functions, similar to that of the ''Escherichia coli'' BglF protein. J. Bacteriol. 177: 1527-1535.  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
 
# Krüger, S., Gertz, S. & Hecker, M. Transcriptional analysis of ''bglPH'' expression in ''Bacillus subtilis'': Evidence for two distinct pathways mediating carbon catabolite repression. J. Bacteriol. 178, 2637-2644 (1996). [http://www.ncbi.nlm.nih.gov/sites/entrez/8626332 PubMed]
 
# Krüger, S. and Hecker, M. (1995) Regulation of the putative ''bglPH'' operon for aryl-ß-glycoside utilization in ''Bacillus subtilis''. J. Bacteriol. 177, 5590-5597. [http://www.ncbi.nlm.nih.gov/sites/entrez/7559347 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:51, 16 April 2009

  • Description: cell wall-associated protein precursor

Gene name wapA
Synonyms
Essential no
Product cell wall-associated protein precursor
Function unknown
MW, pI 258 kDa, 6.131
Gene length, protein length 7002 bp, 2334 aa
Immediate neighbours yxxG, yxxF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
WapA context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  2. Serizawa M, Kodama K, Yamamoto H, Kobayashi K, Ogasawara N, Sekiguchi J. (2005) Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses. Biosci. Biotechnol. Biochem. 69: 2155-2169. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed