Difference between revisions of "PGP570"
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| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:03, 3 June 2013
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
based on pWH844
in E. coli: ampicillin resistance
unpublished
