Difference between revisions of "PGP172"
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| − | vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein | + | vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein |
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
| Line 13: | Line 13: | ||
based on pET3C | based on pET3C | ||
| − | similar plasmid: [[pGP574]] | + | similar [[plasmid]]: [[pGP574]] |
sequencing primer: | sequencing primer: | ||
Revision as of 11:59, 16 April 2009
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) Mycoplasma pneumoniae HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. PubMed
