Difference between revisions of "Sandbox"

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* '''Description:''' RNA polymerase [[sigma factor]] SigD <br/><br/>
+
* '''Description:''' required for methylation of methyl-accepting chemotaxis proteins by CheR,  enhances phosphatase activity of CheC <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sigD''
+
|''cheD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''flaB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylxK ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase [[sigma factor]] SigD
+
|style="background:#ABCDEF;" align="center"| '''Product''' || CheC activity modulator
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of flagella, motility, chemotaxis and autolysis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || motility, chemotaxis
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.167  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.347  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 762 bp, 254 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 498 bp, 166 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheD]]'', ''[[swrB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheC]]'', ''[[sigD]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sigD_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:cheD_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10751]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10750]
  
 
=== Additional information===
 
=== Additional information===
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* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 
=== Genes/ operons controlled by SigD ===
 
''[[dltA]]-[[dltB]]-[[dltC]]-[[dltD]]-[[dltE]]'', ''[[cheV]]'', ''[[degR]]'', ''[[epr]]'', ''[[hag]]'', ''[[lytA]]-[[lytB]]-[[lytC]]'', ''[[lytD]]'', ''[[lytF]]'', ''[[tlpA]]-[[mcpA]]'', ''[[mcpB]]'', ''[[mcpC]]'', ''[[motA]]-[[motB]]'', ''[[nfrA]]-[[ywcH]]''
 
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 83: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16470]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16460]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 93: Line 90:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigD]]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 99: Line 96:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+
* '''two-hybrid system:'''  
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 19:48, 15 April 2009

  • Description: required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC

Gene name cheD
Synonyms ylxK
Essential no
Product CheC activity modulator
Function motility, chemotaxis
MW, pI 17 kDa, 9.347
Gene length, protein length 498 bp, 166 aa
Immediate neighbours cheC, sigD
Hier soll was neues rein
Genetic context
CheD context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed