Difference between revisions of "Sandbox"

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* '''Description:''' DNA polymerase X, involved in DNA repair <br/><br/>
+
* '''Description:''' probable DNA mismatch repair protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''polX''
+
|''mutSB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yshC''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yshD ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA polymerase X
+
|style="background:#ABCDEF;" align="center"| '''Product''' || probable DNA mismatch repair protein
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 63 kDa, 5.308  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 87 kDa, 5.79  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1710 bp, 570 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2355 bp, 785 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutSB]]'', ''[[yshB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yshE]]'', ''[[polX]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yshC_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:mutSB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12327]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12328]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' template-dependent DNA polymerase, fills single nucleotide gaps [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed], has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates [http://www.ncbi.nlm.nih.gov/sites/entrez/18776221 PubMed]
+
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' DNA polymerase X family
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' monomeric [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed]
+
* '''Interactions:'''
  
 
* '''Localization:'''
 
* '''Localization:'''
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28590]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28580]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 114: Line 116:
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
  
[[Margarita Salas]], Madrid, Spain [http://www.cbm.uam.es/mkfactory.esdomain/webs/CBMSO/plt_LineasInvestigacion.aspx?IdObjeto=28 link]
+
[[Alessandra Albertini]], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
# Banos et al.  (2008) Characterization of a  Bacillus subtilis 64 kDa DNA polymerase X potentially involved in DNA repair.  ''J. Mol. Biol.'' '''384:''' 1019-1028. [http://www.ncbi.nlm.nih.gov/sites/entrez/18938175 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
# Banos et al.  (2008) Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase.  ''Nucl. Acids Res.'' '''36:''' 5736-5749. [http://www.ncbi.nlm.nih.gov/sites/entrez/18776221 PubMed]
 

Revision as of 18:22, 15 April 2009

  • Description: probable DNA mismatch repair protein

Gene name mutSB
Synonyms yshD
Essential no
Product probable DNA mismatch repair protein
Function DNA repair
MW, pI 87 kDa, 5.79
Gene length, protein length 2355 bp, 785 aa
Immediate neighbours yshE, polX
Hier soll was neues rein
Genetic context
MutSB context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Alessandra Albertini, University of Pavia, Italy homepage

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed