Difference between revisions of "Sandbox"

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* '''Description:''' phosphatidylglycerophosphate synthase <br/><br/>
+
* '''Description:''' gamma-DL-glutamyl hydrolase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pgsA''
+
|''pgdS''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ymfN ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ywtD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylglycerophosphate synthase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || gamma-DL-glutamyl hydrolase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
+
|style="background:#ABCDEF;" align="center"|'''Function''' || polyglutamic acid degradation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.163  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.52  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1239 bp, 413 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rodZ]]'', ''[[cinA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtE]]'', ''[[capE]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|-
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtD_context.gif]]
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pgsA_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 31: Line 29:
 
__TOC__
 
__TOC__
  
<br/><br/><br/><br/>
+
<br/><br/>
  
 
=The gene=
 
=The gene=
Line 40: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtD.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11373]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12535]
  
 
=== Additional information===
 
=== Additional information===
Line 76: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:'''
+
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 84: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16920]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU35860]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 122: Line 118:
 
=References=
 
=References=
  
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 +
# Suzuki & Tahara (2003) Characterization of the ''Bacillus subtilis ywtD'' gene, whose product is involved in gamma-polyglutamic acid degradation. ''J. Bacteriol.'' '''185:''' 2379-2382. [http://www.ncbi.nlm.nih.gov/sites/entrez/12644511 PubMed]

Revision as of 17:29, 15 April 2009

  • Description: gamma-DL-glutamyl hydrolase

Gene name pgdS
Synonyms ywtD
Essential no
Product gamma-DL-glutamyl hydrolase
Function polyglutamic acid degradation
MW, pI 45 kDa, 8.52
Gene length, protein length 1239 bp, 413 aa
Immediate neighbours ywtE, capE
Hier soll was neues rein
Genetic context
YwtD context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  2. Suzuki & Tahara (2003) Characterization of the Bacillus subtilis ywtD gene, whose product is involved in gamma-polyglutamic acid degradation. J. Bacteriol. 185: 2379-2382. PubMed