Difference between revisions of "Sandbox"

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* '''Description:''' spore coat protein <br/><br/>
+
* '''Description:''' production of glutamyl-tRNA(Gln) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yeeK''
+
|''gatB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yerN ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein
+
|style="background:#ABCDEF;" align="center"| '''Product''' || glutamyl-tRNA(Gln) amidotransferase (sununit B)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || translation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 15 kDa, 6.852  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.898  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 435 bp, 145 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1428 bp, 476 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yeeI]]'', ''[[yezE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gatA]]'', ''[[yerO]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yeeK_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:gatB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
+
 
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 +
 
 
=== Database entries ===
 
=== Database entries ===
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12825]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12840]
  
 
=== Additional information===
 
=== Additional information===
Line 72: Line 74:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06850]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06690]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
 
[[CotE]], [[SafA]], and [[SpoVID]] are required for proper localization, clolocalizes with [[YabG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''yeeK'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''Operon:'''  
  
* '''[[Sigma factor]]:''' [[SigK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' expression startts 5 hrs after the onset of sporulation, activated by [[GerE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 120:
 
=References=
 
=References=
  
# Takamatsu et al. (2009) Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy''J. Bacteriol.'' '''191:''' 1220-1229. [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:52, 15 April 2009

  • Description: production of glutamyl-tRNA(Gln)

Gene name gatB
Synonyms yerN
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase (sununit B)
Function translation
MW, pI 53 kDa, 4.898
Gene length, protein length 1428 bp, 476 aa
Immediate neighbours gatA, yerO
Hier soll was neues rein
Genetic context
GatB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed