Difference between revisions of "Sandbox"

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−
* '''Description:''' required for spore maturation <br/><br/>
+
* '''Description:''' anti-SigF/ protein serine kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''spoVAF''
+
|''spoIIAB''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || anti-SigF/ protein serine kinase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || spore maturation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of SigF activity
 +
septation; phosphorylation and inactivation of SpoIIAA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 6.211  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 4.383  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1476 bp, 492 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 438 bp, 146 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lysA]]'', ''[[spoVAE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigF]]'', ''[[spoIIAA]]''
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/spoVAF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/spoIIAB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/spoVAF_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/spoIIAB_protein.txt Protein sequence]'''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
+
|colspan="2" | '''Genetic context''' <br/> [[Image:spoIIAB_context.gif]]
−
|-
 
−
|colspan="2" | '''Genetic context''' <br/> [[Image:spoVAF_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoVAABCDEF-lysA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10508]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10297]
  
 
=== Additional information===
 
=== Additional information===
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' gerABKA family
+
* '''Protein family:''' anti-sigma-factor family
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23390]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23460]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[spoVAA]]-[[spoVAB]]-[[spoVAC]]-[[spoVAD]]-[[spoVAE]]-[[spoVAF]]-[[lysA]]''
+
* '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
 +
''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
  
−
* '''[[Sigma factor]]:''' [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1903432 PubMed]
+
* '''[[Sigma factor]]:''' ''[[dacF]]'': [[SigF]], [[SigG]]
 +
''[[spoIIA]]'': [[SigH]]
  
−
* '''Regulation:'''  
+
* '''Regulation:''' ''[[dacF]]'': expressed during sporulation, ''[[spoIIAA]]'': expressed early during sporulation
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 05:23, 12 April 2009

  • Description: anti-SigF/ protein serine kinase

Gene name spoIIAB
Synonyms
Essential no
Product anti-SigF/ protein serine kinase
Function control of SigF activity

septation; phosphorylation and inactivation of SpoIIAA

MW, pI 16 kDa, 4.383
Gene length, protein length 438 bp, 146 aa
Immediate neighbours sigF, spoIIAA
Gene sequence (+200bp) Protein sequence
Genetic context
SpoIIAB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: anti-sigma-factor family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

spoIIAA-spoIIAB-sigF

spoIIA: SigH

  • Regulation: dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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