Difference between revisions of "Sandbox"

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* '''Description:''' tRNA isopentenylpyrophosphate transferase <br/><br/>
+
* '''Description:''' ribonucleoside-diphosphate reductase (major subunit) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''miaA''
+
|''nrdF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || tRNA isopentenylpyrophosphate transferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || ribonucleoside-diphosphate reductase (major subunit)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
+
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 6.002  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 4.66  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 942 bp, 314 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ymaG]]'', ''[[hfq]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/miaA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrdF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/miaA_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrdF_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
+
|colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]]
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:miaA_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 39:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nrdIEF-ymaB.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12617]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11405]
  
 
=== Additional information===
 
=== Additional information===
Line 57: Line 57:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' IPP transferase family
+
* '''Protein family:''' ribonucleoside diphosphate reductase small chain family
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[YosP]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 83: Line 83:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU17330]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU17390]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 04:48, 12 April 2009

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdF
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
MW, pI 38 kDa, 4.66
Gene length, protein length 987 bp, 329 aa
Immediate neighbours nrdE, ymaB
Gene sequence (+200bp) Protein sequence
Genetic context
NrdF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ribonucleoside diphosphate reductase small chain family
  • Paralogous protein(s): YosP

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed