Difference between revisions of "Sandbox"

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−
* '''Description:''' unknown <br/><br/>
+
* '''Description:''' small acid-soluble spore protein (minor) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''yabG''
+
|''sspF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (minor)
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.143  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 183 bp, 61 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ksgA]]'', ''[[veg]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yabG_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sspF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yabG_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sspF_protein.txt Protein sequence]'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:yabG_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yabG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspF.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10106]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10108]
  
 
=== Additional information===
 
=== Additional information===
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:''' peptidase U57 family
+
* '''Protein family:''' alpha/beta-type SASP family
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 73: Line 73:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00430]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00450]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
−
colocalizes in the spore coat with [[YeeK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''yabG'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''Operon:''' ''sspF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
−
* '''[[Sigma factor]]:''' [[SigK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''[[Sigma factor]]:''' [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
−
* '''Regulation:''' expression starts four hours after onset of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
* '''Regulation:''' switched on during stage  III of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 120: Line 119:
 
=References=
 
=References=
  
−
# Takamatsu ''et al''. (2000) The ''Bacillus subtilis yabG'' gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.''J. Bacteriol.'' '''182:''' 1883-1888. [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
+
# Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''176:''' 333-348. [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]

Revision as of 02:53, 12 April 2009

  • Description: small acid-soluble spore protein (minor)

Gene name sspF
Synonyms
Essential no
Product small acid-soluble spore protein (minor)
Function protection of spore DNA
MW, pI 6 kDa, 9.647
Gene length, protein length 183 bp, 61 aa
Immediate neighbours veg, ispE
Gene sequence (+200bp) Protein sequence
Genetic context
SspF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: alpha/beta-type SASP family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: switched on during stage III of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis. J. Mol. Biol. 176: 333-348. PubMed
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