Difference between revisions of "Sandbox"

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* '''Description:''' trigger enzyme: fructose-specific phosphotransferase system, EIIB component <br/><br/>
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* '''Description:''' fructose-specific phosphotransferase system, EIIC component <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''levE''
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|''levF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacL ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacL ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: fructose-specific phosphotransferase system, EIIB component
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|style="background:#ABCDEF;" align="center"| '''Product''' || fructose-specific phosphotransferase system, <br/>EIIC component
−
 
 
−
fructose-specific enzyme IIB (EC 2.7.1.69)
 
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation, control of LevR activity
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|style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.444  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 4.395  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 486 bp, 162 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[levF]]'', ''[[levD]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[levG]]'', ''[[levE]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levE_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levE_protein.txt Protein sequence]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/levF_protein.txt Protein sequence]'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:levE_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:levF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
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<br/><br/>
+
<br/><br/><br/>
  
 
=The gene=
 
=The gene=
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10317]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10318]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27060]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27050]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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# Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of ''Bacillus subtilis '' includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
 
# Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of ''Bacillus subtilis '' includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
−
# Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the ''Bacillus subtilis'' transcriptional activator LevR. Mol. Microbiol. 28: 293-303. [http://www.ncbi.nlm.nih.gov/sites/entrez/9622354  PubMed]
 
−
# Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T. 1998. Crystal structure of the IIB subunit of a fructose permease (IIBLev) from ''Bacillus subtilis''. J. Mol. Biol. 276:591-602. [http://www.ncbi.nlm.nih.gov/sites/entrez/9551099 PubMed]
 
−
# Seip, S., R. Lanz, R. Gutknecht, K. Flükiger, and B. Erni. 1997. The fructose transporter of Bacillus subtilis encoded by the lev operon. Backbone assignment and secondary structure of the IIBLev subunit. Eur. J. Biochem. 243: 306-314. [http://www.ncbi.nlm.nih.gov/sites/entrez/9030753 PubMed]
 
−
# Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. [http://www.ncbi.nlm.nih.gov/sites/entrez/9033408 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 11:58, 6 April 2009

  • Description: fructose-specific phosphotransferase system, EIIC component

Gene name levF
Synonyms sacL
Essential no
Product fructose-specific phosphotransferase system,
EIIC component
Function fructose uptake and phosphorylation
MW, pI 27 kDa, 4.395
Gene length, protein length 807 bp, 269 aa
Immediate neighbours levG, levE
Gene sequence (+200bp) Protein sequence
Genetic context
LevF context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
  • Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  1. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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