Difference between revisions of "Sandbox"

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* '''Description:''' acetyl-CoA synthetase <br/><br/>
+
* '''Description:''' phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acsA''
+
|''phrC''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA synthetase)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of acetate, fatty acids
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 4 kDa, 8.16  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1716 bp, 572 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 120 bp, 40 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tyrS]]'', ''[[acuA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rapC]]'', ''[[yclM]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/acsA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/phrC_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/acsA_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/phrC_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:acsA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:phrC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 42: Line 42:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acsA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rapC-phrC.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10370]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11959]
  
 
=== Additional information===
 
=== Additional information===
Line 65: Line 65:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:''' acetylated on Lys-549 by [[AcuA]], this results in inactivation  [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed], deacetylated by [[SrtN]] and [[AcuC]] deacetylates (and thereby activates) [[AcsA]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
+
* '''Modification:'''
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 81: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29680]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03780]
  
* '''E.C. number:''' 6.2.1.1
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
Line 120: Line 120:
  
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
# Gardner JG, Escalante-Semerena JC (2008) Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in ''Bacillus subtilis''. ''J. Bacteriol.'' '''190:''' 5132-5136. [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed]
 
# Gardner JG, Escalante-Semerena JC (2009) In ''Bacillus subtilis'', the sirtuin protein deacetylase, encoded by the ''srtN'' gene (formerly ''yhdZ''), and functions encoded by the ''acuABC'' genes control the activity of acetyl coenzyme A synthetase. ''J. Bacteriol.'' '''191:''' 1749-1755. [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 10:02, 6 April 2009

  • Description: phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)

Gene name phrC
Synonyms
Essential no
Product phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
Function control of sporulation initiation
MW, pI 4 kDa, 8.16
Gene length, protein length 120 bp, 40 aa
Immediate neighbours rapC, yclM
Gene sequence (+200bp) Protein sequence
Genetic context
PhrC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by glucose (4.5-fold) (CcpA) PubMed
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed