Difference between revisions of "Sandbox"

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* '''Description:''' RNA polymerase forespore-specific (early) sigma factor SigF <br/><br/>
+
* '''Description:''' write here <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sigF''
+
|''gerM''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIAC ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase forespore-specific (early) sigma factor SigF
+
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (late mother cell)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || germination (cortex hydrolysis) and sporulation (stage II, multiple polar septa)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.078  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.683  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVAA]]'', ''[[spoIIAB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rph]]'', ''[[racE]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sigF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gerM_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sigF_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gerM_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sigF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:gerM_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gerM.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10298]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10356]
  
 
=== Additional information===
 
=== Additional information===
Line 58: Line 58:
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 
=== Genes controlled by SigF ===
 
 
''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'','' [[pbpF]]'', ''[[yphA]]-[[seaA]]''
 
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 85: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23450]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28380]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 93: Line 89:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
+
* '''Operon:'''  
''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
 
  
* '''Sigma factor:''' ''[[dacF]]'': [[SigF]], [[SigG]]
+
* '''Sigma factor:'''  
''[[spoIIA]]'': [[SigH]]
 
  
* '''Regulation:''' repressed by Casamino Acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] , ''[[dacF]]'': expressed during sporulation, ''[[spoIIAA]]'': expressed early during sporulation
+
* '''Regulation:''' repressed by Casamino Acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
  
* '''Regulatory mechanism:''' ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]]
+
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =

Revision as of 11:12, 31 March 2009

  • Description: write here

Gene name gerM
Synonyms
Essential no
Product
Function germination (cortex hydrolysis) and sporulation (stage II, multiple polar septa)
MW, pI 39 kDa, 4.683
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours rph, racE
Gene sequence (+200bp) Protein sequence
Genetic context
GerM context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by Casamino Acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed