Mbl

• Description: cell-shape determining protein
  
  

Gene name	mbl
Synonyms
Essential	no
Product	MreB-like protein
Function	cell-shape determination
MW, pI	35 kDa, 5.669
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	flhO, spoIIID
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

non-viable in the presence of low Mg(2+), readily accumulate rsgI suppressor mutants PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): MreB, MreBH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MreB-Mbl, Mbl-MreBH PubMed

• Localization: forms helical structures close to the inner surface of the cytoplasmic membrane PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in the Jeff Errington and Peter Graumann labs

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

1. Carballido-Lopez, R., Formstone, A., Li, Y., Ehrlich, S. D., and Errington, J. (2006) Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE. Dev. Cell 11: 399-409. PubMed
2. Defeu Soufo, H. J., and P. Graumann. 2006. Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the funtion of MreB. Mol. Microbiol. 62: 1340-1356. PubMed
3. Schirner K, Errington J. (2009) Cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in Bacillus subtilis. J. Bacteriol. 191: 1404-1413. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed