Difference between revisions of "PonA"
| Line 75: | Line 75: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' localizes to the spore septum during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary] | + | * '''Localization:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed] , localizes to the spore septum during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary] |
=== Database entries === | === Database entries === | ||
| Line 123: | Line 123: | ||
=References= | =References= | ||
| + | # Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed] | ||
# Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in ''Bacillus subtilis'' by recruitment of PBP1 to the MreB helix. ''Mol. Microbiol.'' '''71:''' 1131-1144. [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] | # Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in ''Bacillus subtilis'' by recruitment of PBP1 to the MreB helix. ''Mol. Microbiol.'' '''71:''' 1131-1144. [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
Revision as of 14:51, 25 March 2009
- Description: penicillin-binding proteins 1A/1B
| Gene name | ponA |
| Synonyms | |
| Essential | no |
| Product | penicillin-binding proteins 1A/1B |
| Function | bifunctional glucosyltransferase/ transpeptidase |
| MW, pI | 99 kDa, 4.752 |
| Gene length, protein length | 2742 bp, 914 aa |
| Immediate neighbours | recU, ypoC |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: membrane associated PubMed , localizes to the spore septum during sporulation Link to a summary
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation: constitutive during vegetative growth Link to a summary
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
- Antibody:
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Your additional remarks
References
- Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
- Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. Mol. Microbiol. 71: 1131-1144. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
