Difference between revisions of "PGP380"
| Line 8: | Line 8: | ||
in ''E. coli'': ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
| − | in | + | in ''B. subtilis'': erythromycin resistance |
based on [[pBQ200]] | based on [[pBQ200]] | ||
Revision as of 11:23, 16 April 2009
Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology
constructed in Stülke lab
in E. coli: ampicillin resistance
in B. subtilis: erythromycin resistance
based on pBQ200
similar vector: pGP382
sequencing primer:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed
