Difference between revisions of "PGP172"
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[[File:PGP172.jpeg|250px|thumb|right|'''pGP172: click to enlarge''']] | [[File:PGP172.jpeg|250px|thumb|right|'''pGP172: click to enlarge''']] | ||
| + | [[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']] | ||
pGP172 | pGP172 | ||
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similar plasmid: [[pGP574]] | similar plasmid: [[pGP574]] | ||
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| + | sequencing primer: | ||
| + | *'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | ||
| + | *'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| + | |||
Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) ''Mycoplasma pneumoniae'' HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. [http://www.ncbi.nlm.nih.gov/sites/entrez/14717704 PubMed] | Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) ''Mycoplasma pneumoniae'' HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. [http://www.ncbi.nlm.nih.gov/sites/entrez/14717704 PubMed] | ||
Revision as of 13:54, 18 March 2009
pGP172
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
similar plasmid: pGP574
sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) Mycoplasma pneumoniae HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. PubMed
