Difference between revisions of "PGP380"
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[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']] | [[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']] | ||
| + | [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | ||
Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology | Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology | ||
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similar vector: [[pGP382]] | similar vector: [[pGP382]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘ | ||
| + | *'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | ||
| + | |||
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed] | Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed] | ||
Revision as of 12:29, 18 March 2009
Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology
constructed in Stülke lab
in E. coli: ampicillin resistance
in B. subtilis: erythromycin resistance
based on pBQ200
similar vector: pGP382
sequencing primer:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed
