Difference between revisions of "Spizizen minimal medium"
(Created page with "* Spizizen medium (SM) is a popular minimal medium for the cultivation of ''B. subtilis''. ==SM medium== 200 ml 5x SM base medium 10 ml 100x Trace element solution 10 ml 100...") |
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==SM medium== | ==SM medium== | ||
| − | 200 ml 5x SM base medium | + | *200 ml 5x SM base medium |
| − | 10 ml 100x Trace element solution | + | *10 ml 100x Trace element solution |
| − | 10 ml 100x Fe-Citrate-Solution | + | *10 ml 100x Fe-Citrate-Solution |
| − | 10 ml Glucose (50 %) | + | *10 ml Glucose (50 %) |
| − | 10 ml Tryptophan (5 mg/ml) for B. subtilis 168 | + | *10 ml Tryptophan (5 mg/ml) for B. subtilis 168 |
| − | Add N-source solution to final concentration of 1 % | + | *Add N-source solution to final concentration of 1 % |
| − | Ad 1,000 ml with deionized H2O | + | *Ad 1,000 ml with deionized H2O |
==5x SM base medium== | ==5x SM base medium== | ||
| − | 175 g K2HPO4 | + | *175 g K2HPO4 |
| − | 75 g KH2PO4 | + | *75 g KH2PO4 |
| − | 12.5 g Na3-Citrate ∙ 2 H2O | + | *12.5 g Na3-Citrate ∙ 2 H2O |
| − | 2.5 g MgSO4 ∙ 7 H2O | + | *2.5 g MgSO4 ∙ 7 H2O |
| − | Ad 2,000 ml with deionized H2O | + | *Ad 2,000 ml with deionized H2O |
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| + | ==N-source solutions== | ||
| + | *Glutamine 4 % (do not autoclave) | ||
| + | *Glutamate 40 % | ||
| + | *(NH4)2 SO4, final concentration 0.2% | ||
==100x Trace element solution== | ==100x Trace element solution== | ||
| − | 0.55 g CaCl2 / 0.735 g CaCl2 ∙ 2 H2O | + | *0.55 g CaCl2 / 0.735 g CaCl2 ∙ 2 H2O |
| − | 0.1 g MnCl2 ∙ 4 H2O | + | *0.1 g MnCl2 ∙ 4 H2O |
| − | 0.17 g ZnCl2 | + | *0.17 g ZnCl2 |
| − | 0.033 g CuCl2 ∙ 2 H2O / 0.043 g CuCl2 ∙ 6 H2O | + | *0.033 g CuCl2 ∙ 2 H2O / 0.043 g CuCl2 ∙ 6 H2O |
| − | 0.06 g CoCl2 ∙ 6 H2O | + | *0.06 g CoCl2 ∙ 6 H2O |
| − | 0.06 g Na2MoO4 ∙ 2 H2O | + | *0.06 g Na2MoO4 ∙ 2 H2O |
| − | Ad 1,000 ml with deionized H2O | + | *Ad 1,000 ml with deionized H2O |
| − | Do not autoclave, use sterile filtration! | + | *Do not autoclave, use sterile filtration! |
| − | 100x Fe-Citrate-Solution | + | ==100x Fe-Citrate-Solution== |
| − | 0.0135 g FeCl3 ∙ 6 H2O | + | *0.0135 g FeCl3 ∙ 6 H2O |
| − | 0.1 g Na3-Citrate ∙ 3 H2O | + | *0.1 g Na3-Citrate ∙ 3 H2O |
| − | Ad 100 ml with deionized H2O | + | *Ad 100 ml with deionized H2O |
| − | Do not autoclave, use sterile filtration! | + | *Do not autoclave, use sterile filtration! |
Latest revision as of 12:04, 10 August 2016
- Spizizen medium (SM) is a popular minimal medium for the cultivation of B. subtilis.
Contents
SM medium
- 200 ml 5x SM base medium
- 10 ml 100x Trace element solution
- 10 ml 100x Fe-Citrate-Solution
- 10 ml Glucose (50 %)
- 10 ml Tryptophan (5 mg/ml) for B. subtilis 168
- Add N-source solution to final concentration of 1 %
- Ad 1,000 ml with deionized H2O
5x SM base medium
- 175 g K2HPO4
- 75 g KH2PO4
- 12.5 g Na3-Citrate ∙ 2 H2O
- 2.5 g MgSO4 ∙ 7 H2O
- Ad 2,000 ml with deionized H2O
N-source solutions
- Glutamine 4 % (do not autoclave)
- Glutamate 40 %
- (NH4)2 SO4, final concentration 0.2%
100x Trace element solution
- 0.55 g CaCl2 / 0.735 g CaCl2 ∙ 2 H2O
- 0.1 g MnCl2 ∙ 4 H2O
- 0.17 g ZnCl2
- 0.033 g CuCl2 ∙ 2 H2O / 0.043 g CuCl2 ∙ 6 H2O
- 0.06 g CoCl2 ∙ 6 H2O
- 0.06 g Na2MoO4 ∙ 2 H2O
- Ad 1,000 ml with deionized H2O
- Do not autoclave, use sterile filtration!
100x Fe-Citrate-Solution
- 0.0135 g FeCl3 ∙ 6 H2O
- 0.1 g Na3-Citrate ∙ 3 H2O
- Ad 100 ml with deionized H2O
- Do not autoclave, use sterile filtration!
