Fra

• Description: frataxin-like protein, required for the biosynthesis of iron-sulfur clusters
  
  

Gene name	fra
Synonyms	ydhG
Essential	no
Product	frataxin-like protein
Function	intracellular iron channeling, biosynthesis of iron-sulfur clusters
Gene expression levels in SubtiExpress: fra
Interactions involving this protein in SubtInteract: Fra
Metabolic function and regulation of this protein in SubtiPathways: Fra
MW, pI	14 kDa, 5.377
Gene length, protein length	369 bp, 123 aa
Immediate neighbours	phoB, ydhH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

iron metabolism

The gene

Basic information

• Locus tag: BSU05750

Phenotypes of a mutant

• severe growth phenotype associated with a broadly disturbed iron homeostasis PubMed

Database entries

• BsubCyc: BSU05750

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transfers iron onto the B. subtilis SUF system (SufU-SufS) for iron-sulfur cluster biosynthesis PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Fe(2+) is bound with high affinity PubMed

• Effectors of protein activity:

• Interactions:
  • the protein is active as a dimer PubMed
  • (Fra)2-SufU, requires iron PubMed
  • (Fra)-HemH, requires iron, Kd of 1.63 ± 0.02 μM PubMed

• Localization:

Database entries

• BsubCyc: BSU05750

• Structure: 2OC6

• UniProt: Q797E6

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: fra PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 178 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 451 PubMed

Biological materials

• Mutant: available in Mohamed Marahiel's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Andreas Mielcarek, Bastian Blauenburg, Marcus Miethke, Mohamed A Marahiel
Molecular insights into frataxin-mediated iron supply for heme biosynthesis in Bacillus subtilis.
PLoS One: 2015, 10(3);e0122538
[PubMed:25826316] [WorldCat.org] [DOI] (I e)

Alexander G Albrecht, Hannes Landmann, David Nette, Olaf Burghaus, Florian Peuckert, Andreas Seubert, Marcus Miethke, Mohamed A Marahiel
The frataxin homologue Fra plays a key role in intracellular iron channeling in Bacillus subtilis.
Chembiochem: 2011, 12(13);2052-61
[PubMed:21744456] [WorldCat.org] [DOI] (I p)

Wenbin Qi, J A Cowan
A structural and functional homolog supports a general role for frataxin in cellular iron chemistry.
Chem Commun (Camb): 2010, 46(5);719-21
[PubMed:20087498] [WorldCat.org] [DOI] (I p)