Difference between revisions of "SacT"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[DnaAregulon]]}} | ||
=The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''= | =The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''= | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}} | + | * '''Operon:''' |
| + | ** ''[[ywcI]]-[[sacT]]'' {{PubMed|22383849}} | ||
| + | ** ''[[sacT]]'' {{PubMed|9353933}} | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[DnaA]]: transcription repression {{PubMed|26020636}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 </pubmed> | + | <pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 26020636 22383849</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:33, 1 June 2015
- Description: transcriptional antiterminator for the sacP-sacA-ywdA operon
| Gene name | sacT |
| Synonyms | ipa-47d |
| Essential | no |
| Product | transcriptional antiterminator |
| Function | regulation of sucrose utilization |
| Gene expression levels in SubtiExpress: sacT | |
| Interactions involving this protein in SubtInteract: SacT | |
| Metabolic function and regulation of this protein in SubtiPathways: sacT | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 828 bp, 276 aa |
| Immediate neighbours | ywcJ, ywcI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
| Expression at a glance PubMed 500px | |
Contents
Categories containing this gene/protein
utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins
This gene is a member of the following regulons
The SacT regulon: sacP-sacA-ywdA
The gene
Basic information
- Locus tag: BSU38070
Phenotypes of a mutant
Database entries
- BsubCyc: BSU38070
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU38070
- Structure:
- UniProt: P26212
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- repressed by casamino acids PubMed
- Additional information:
Biological materials
- Mutant: GP429 (spc), available in Jörg Stülke's lab
- Expression vector:
- for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Jörg Stülke's lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Jörg Stülke's lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Jörg Stülke's lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Jörg Stülke's lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Jörg Stülke's lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Jörg Stülke's lab
- for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Jörg Stülke's lab
- for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Jörg Stülke's lab
- for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion: GP1227 (spc, based on pGP1870), available in Jörg Stülke's lab
- YFP fusion: GP1231 (spc, based on pGP1871), available in Jörg Stülke's lab
- FLAG-tag construct: GP1223 (spc, based on pGP1331), available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Janet L Smith, Alan D Grossman
In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA.
PLoS Genet: 2015, 11(5);e1005258
[PubMed:26020636]
[WorldCat.org]
[DOI]
(I e)
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
Sebastian Hübner, Nathalie Declerck, Christine Diethmaier, Dominique Le Coq, Stephane Aymerich, Jörg Stülke
Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family.
Nucleic Acids Res: 2011, 39(10);4360-72
[PubMed:21278164]
[WorldCat.org]
[DOI]
(I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561]
[WorldCat.org]
[DOI]
(P p)
S Aymerich, M Steinmetz
Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family.
Proc Natl Acad Sci U S A: 1992, 89(21);10410-4
[PubMed:1279678]
[WorldCat.org]
[DOI]
(P p)
M Arnaud, P Vary, M Zagorec, A Klier, M Debarbouille, P Postma, G Rapoport
Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity.
J Bacteriol: 1992, 174(10);3161-70
[PubMed:1577686]
[WorldCat.org]
[DOI]
(P p)
M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394]
[WorldCat.org]
[DOI]
(P p)
