Difference between revisions of "MifM"
Line 88: | Line 88: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
− | ** the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel {{PubMed|19779460}} | + | ** the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel ([[RplD]], [[RplV]]) {{PubMed|25903689,19779460}} |
** [[SpoIIIJ]]-[[MifM]] {{PubMed|22864117}} | ** [[SpoIIIJ]]-[[MifM]] {{PubMed|22864117}} | ||
+ | ** [[RplD]]-[[MifM]] {{PubMed|25903689}} | ||
+ | ** [[RplV]]-[[MifM]] {{PubMed|25903689}} | ||
+ | ** [[23S rRNA]]-[[MifM]] {{PubMed|25903689}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
Line 143: | Line 146: | ||
=References= | =References= | ||
− | <pubmed> 19779460 21383133 20525796 22456704 22383849 22864117 25313395 </pubmed> | + | <pubmed> 19779460 21383133 20525796 22456704 22383849 22864117 25313395 25903689</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 14:52, 24 April 2015
- Description: ribosome-nascent chain sensor of membrane protein biogenesis
Gene name | mifM |
Synonyms | yqzJ |
Essential | no |
Product | sensor of SpoIIIJ activity |
Function | control of yidC2 translation |
Gene expression levels in SubtiExpress: mifM | |
Interactions involving this protein in SubtInteract: MifM | |
MW, pI | 11 kDa, 5.829 |
Gene length, protein length | 285 bp, 95 aa |
Immediate neighbours | polY1, yidC2 |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
translation, membrane proteins
This gene is a member of the following regulons
The MifM regulon: yidC2
The gene
Basic information
- Locus tag: BSU23880
Phenotypes of a mutant
Database entries
- BsubCyc: BSU23880
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- membrane PubMed
Database entries
- BsubCyc: BSU23880
- Structure:
- UniProt: Q7WY64
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for mifM PubMed
- MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
- elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References