Difference between revisions of "YodF"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 128: Line 128:
 
** available in [[Erhard Bremer]]'s lab {{PubMed|24142252}}
 
** available in [[Erhard Bremer]]'s lab {{PubMed|24142252}}
 
** GP1887 (kan) available in [[Jörg Stülke]]'s lab
 
** GP1887 (kan) available in [[Jörg Stülke]]'s lab
** GP1889 (''ytnA'' replaced by a spec cassette and ''yodF'' replaced by a kan cassette) available in [[Jörg Stülke]]'s lab
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 20:13, 19 September 2014

  • Description: similar to amino acid permease

Gene name yodF
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yodF
Metabolic function and regulation of this protein in SubtiPathways:
YodF
MW, pI 53 kDa, 9.682
Gene length, protein length 1488 bp, 496 aa
Immediate neighbours yoyD, ctpA
Sequences Protein DNA DNA_with_flanks
Genetic context
YodF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YodF expression.png















Categories containing this gene/protein

transporters/ other, putative amino acid transporter, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU19580

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • in some database, the protein is annotated as being similar to proline permease, however, it is not involved in proline transport PubMed
  • Protein family: solute:sodium symporter family (SSS family)
  • Paralogous protein(s): YhjB

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by casamino acids PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 445 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 807 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References