tig

tig
168

trigger factor (prolyl isomerase)

Locus
BSU_28230
Molecular weight
47.00 kDa
Isoelectric point
4
Protein length
Gene length
Function
protein folding
Product
trigger factor (prolyl isomerase)
Essential
no
E.C.
5.2.1.8
Synonyms
tig, yzzH

Genomic Context

List of homologs in different organisms, belongs to COG0544 (Galperin et al., 2021)

This gene is a member of the following regulons

Stringent response

Gene
Coordinates
2,886,315 2,887,589
Phenotypes of a mutant
delayed spore germination PubMed
a tig dnaK double mutant exhibits an aberrant twisted and filamentous cell morphology, as well as decreased tolerance to heat and to cell wall active antibiotics and hydrolytic enzymes, indicative of defects in cell wall integrity PubMed
The protein
Catalyzed reaction/ biological activity
[protein]-peptidylproline (=180) --> [protein]-peptidylproline (=0) (according to UniProt)
Protein family
FKBP-type PPIase family (single member, according to UniProt)
PPIase FKBP-type domain (aa 163-248) (according to UniProt)
Structure
2VRH (PDB) (the E. coli trigger factor) PubMed
Modification
phosphorylated on Arg-45, the phosphorylation interferes with Tig binding to ribosomes PubMed
phosphorylated on Arg-90 PubMed
phosphorylated on ser/ thr/ tyr PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
RelA dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
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tig

2025-02-12 07:07:18

ghost

70

53f7095ed528292caaf637168cb2d213ed866408

2549579C371F2DE79092E0D421F6F5BFE732A9FB

Biological materials
Mutant
BKE28230 (tig::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTTCCCTCCAAAAA, downstream forward: _UP4_TAATATGGTGCATAATAATA
BKK28230 (tig::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTGTTTCCCTCCAAAAA, downstream forward: _UP4_TAATATGGTGCATAATAATA
References
Reviews
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Original Publications
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Time of last update: 2025-02-24 19:14:15

Author of last update: Jstuelk