Difference between revisions of "SasB"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}} | * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}} | ||
| + | * a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant acquires suppressor mutations in ''[[guaA]], [[guaB]], [[gmk]]'' or inactivation of ''[[codY]]'' {{PubMed|24682323,24163341}} | ||
=== Database entries === | === Database entries === | ||
Revision as of 17:55, 10 February 2015
- Description: (p)ppGpp synthetase
| Gene name | sasB |
| Synonyms | yjbM |
| Essential | no |
| Product | (p)ppGpp synthetase |
| Function | ppGpp synthesis independent from stringent response |
| Gene expression levels in SubtiExpress: sasB | |
| MW, pI | 24 kDa, 6.708 |
| Gene length, protein length | 633 bp, 211 aa |
| Immediate neighbours | yjbL, nadF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11600
Phenotypes of a mutant
- a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
- a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): SasA
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31611
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: expressed during exponential growth
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 169 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
