DacF

• Description: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacF
Synonyms
Essential	no
Product	penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacF
Metabolic function and regulation of this protein in SubtiPathways: dacF
MW, pI	43 kDa, 9.912
Gene length, protein length	1167 bp, 389 aa
Immediate neighbours	spoIIAA, pupG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon, AbrB regulon

The gene

Basic information

• Locus tag: BSU23480

Database entries

• BsubCyc: BSU23480

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23480

• Structure:

• UniProt: P38422

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacF-spoIIAA-spoIIAB-sigF PubMed

• Expression browser: dacF PubMed

• Sigma factor: SigF PubMed, SigG

• Regulation:
  • repressed by casamino acids PubMed
  • expressed during sporulation in the forespore PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 95 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: