Difference between revisions of "TopA"
| Line 8: | Line 8: | ||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''topI '' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes {{PubMed|25092907}} |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA topoisomerase I | ||
| Line 40: | Line 40: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[DNA condensation/ segregation]]}} | + | {{SubtiWiki category|[[DNA condensation/ segregation]]}}, |
| + | {{SubtiWiki category|[[essential genes]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 52: | Line 53: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * essential {{PubMed|25092907}} | ||
=== Database entries === | === Database entries === | ||
| Line 70: | Line 72: | ||
* '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot) | * '''Protein family:''' prokaryotic type I/III topoisomerase family (according to Swiss-Prot) | ||
| − | * '''Paralogous protein(s):''' [[TopB] | + | * '''Paralogous protein(s):''' [[TopB]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
| Line 138: | Line 140: | ||
=References= | =References= | ||
| − | <pubmed>17114254,21710567 </pubmed> | + | <pubmed>17114254,21710567 25092907</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:26, 6 August 2014
- Description: DNA topoisomerase I
| Gene name | topA |
| Synonyms | topI |
| Essential | yes PubMed |
| Product | DNA topoisomerase I |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: topA | |
| MW, pI | 78 kDa, 9.023 |
| Gene length, protein length | 2073 bp, 691 aa |
| Immediate neighbours | dprA, trmFO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA condensation/ segregation, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16120
Phenotypes of a mutant
- essential PubMed
Database entries
- BsubCyc: BSU16120
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
- Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
- Paralogous protein(s): TopB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- TopA interacts with the RNA polymerase PubMed
Database entries
- BsubCyc: BSU16120
- Structure:
- UniProt: P39814
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: topA (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 324 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 1123 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
