thdF
168
GTP-binding protein, part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA), synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs
Locus
BSU_41020
Molecular weight
50.81 kDa
Isoelectric point
4.64
Function
tRNA modification
Product
part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA)
Essential
no
Synonyms
thdF, mnmE, trmE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0486 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,211,510 4,212,889
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
(s2U34 in tRNA) + GTP + FAD + CH2-THF + NADH --> 5-carboxymethylaminomethyl-2- thiouridine PubMed
Protein family
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)
TrmE-type G domain (aa 221-380) (according to UniProt)
Structure
1XZP (PDB) (from Thermotoga maritima, 44% identity) PubMed
Paralogous protein(s)
cytoplasm (according to Swiss-Prot)
Additional information
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B877 (thdF::erm), available at the NBRP B. subtilis, Japan
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
Invited review: MnmE, a GTPase that drives a complex tRNA modification reaction.
Biopolymers. 2016 Aug; 105(8):568-79. doi:10.1002/bip.22813. PMID:26832457
The universally conserved prokaryotic GTPases.
Microbiology and molecular biology reviews : MMBR. 2011 Sep; 75(3):507-42, second and third pages of table of contents. doi:10.1128/MMBR.00009-11. PMID:21885683
Original Publications
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Time of last update: 2025-01-18 11:04:56
Author of last update: Jstuelk