thdF

thdF
168

GTP-binding protein, part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA), synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs

Locus
BSU_41020
Molecular weight
50.81 kDa
Isoelectric point
4.64
Protein length
Gene length
Function
tRNA modification
Product
part of 5-carboxymethylaminomethyluridine-tRNA synthase complex (with GidA)
Essential
no
Synonyms
thdF, mnmE, trmE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0486 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,211,510  4,212,889
Phenotypes of a mutant
inactivation of ''thdF'' reduces sporulation efficiency to 21.9% that of wild type cells; longer mother cells, some with aberrant septa and forespores PubMed
The protein
Catalyzed reaction/ biological activity
Binds and hydrolyzes GTP and readily exchanges GDP for GTP
(s2U34 in tRNA) + GTP + FAD + CH2-THF + NADH --> 5-carboxymethylaminomethyl-2- thiouridine PubMed
Protein family
TrmE-type G domain (aa 221-380) (according to UniProt)
Structure
1XZP (PDB) (from Thermotoga maritima, 44% identity) PubMed
Paralogous protein(s)
cytoplasm (according to Swiss-Prot)
Additional information
Information on the corresponding protein from E. coli: EcoCyc
MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.
Expression and Regulation
Operons
Description
Regulation
constitutively expressed PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
DnaA: repression, PubMed, in dnaA regulon
Open in new tab

thdFnoc

2024-12-21 13:10:14

ghost

138

97e0c96ef02da18e76bff0ad52ca51aad0c040d6

02D91CE1EAA3DC9BAA9E45D09CC54A749E1D1DD6

Biological materials
Mutant
MGNA-B877 (thdF::erm), available at the NBRP B. subtilis, Japan
BKE41020 (thdF::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTTGTTCACCTCTCTTT,  downstream forward: _UP4_TAATTAAAGGAGGAACTAGA
BKK41020 (thdF::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTTGTTCACCTCTCTTT,  downstream forward: _UP4_TAATTAAAGGAGGAACTAGA
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
Fislage M, Wauters L, Versées W Invited review: MnmE, a GTPase that drives a complex tRNA modification reaction. Biopolymers. 2016 Aug; 105(8):568-79. doi:10.1002/bip.22813. PMID:26832457
Verstraeten N, Fauvart M, Versées W, Michiels J The universally conserved prokaryotic GTPases. Microbiology and molecular biology reviews : MMBR. 2011 Sep; 75(3):507-42, second and third pages of table of contents. doi:10.1128/MMBR.00009-11. PMID:21885683
Original Publications
Loading

66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7

Page visits: 4305

Time of last update: 2025-01-18 11:04:56

Author of last update: Jstuelk