Difference between revisions of "AroF"
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 4162 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 4162 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2080 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 2080 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1814 {{PubMed|21395229}} | ||
| + | |||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3069 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
| − | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 13:32, 17 April 2014
- Description: chorismate synthase
| Gene name | aroF |
| Synonyms | |
| Essential | no |
| Product | chorismate synthase |
| Function | biosynthesis of aromatic amino acids |
| Gene expression levels in SubtiExpress: aroF | |
| Metabolic function and regulation of this protein in SubtiPathways: aroF | |
| MW, pI | 40 kDa, 6.578 |
| Gene length, protein length | 1107 bp, 369 aa |
| Immediate neighbours | aroB, cheR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22710
Phenotypes of a mutant
Database entries
- BsubCyc: BSU22710
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate (according to Swiss-Prot)
- Protein family: acetokinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- phosphorylated on Arg-127 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU22710
- Structure: 2O11 (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)
- UniProt: P31104
- KEGG entry: [2]
- E.C. number: 4.2.3.5
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 4162 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 2080 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1814 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3069 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
