AlaT

• Description: similar to PLP-dependent methionine aminotransferase
  
  

Gene name	alaT
Synonyms	yugH
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: alaT
Metabolic function and regulation of this protein in SubtiPathways: AlaT
MW, pI	42 kDa, 5.106
Gene length, protein length	1158 bp, 386 aa
Immediate neighbours	yugI, alaR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU31400

Database entries

• BsubCyc: BSU31400

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU31400

• Structure:

• UniProt: Q795M6

• KEGG entry: [2]

• E.C. number:

Additional information

The gene is annotated in KEGG as an ortholog of N-succinyldiaminopimelate aminotransferase EC 2.6.1.17. It is annotated in Swiss-Prot as “alanine transaminase”. In MetaCyc the gene is marked as “similar to aspartate aminotransferase”. No literature/experimental evidence supporting the annotation is available. PubMed

Expression and regulation

• Operon: alaR-alaT PubMed

• Expression browser: alaT PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between yugI and alaT PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 143 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 360 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)