Difference between revisions of "CdaS"
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* '''[[Domains]]:''' | * '''[[Domains]]:''' | ||
| − | ** | + | ** N-terminal autoinhibitory domain {{PubMed|24939848}} |
| + | ** C-terminal [[DAC domain]] involved in the synthesis of c-di-AMP {{PubMed|21566650}} | ||
* '''Modification:''' | * '''Modification:''' | ||
| Line 90: | Line 91: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | + | ** forms hexamers (enzymatically inactive) or dimers (active) {{PubMed|24939848}} | |
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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<pubmed> 18714086</pubmed> | <pubmed> 18714086</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>21566650, 23192352 22383849</pubmed> | + | <pubmed>21566650, 23192352 22383849 24939848 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 06:33, 19 June 2014
- Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
| Gene name | cdaS |
| Synonyms | yojJ |
| Essential | no |
| Product | sporulation-specific diadenylate cyclase |
| Function | synthesis of c-di-AMP |
| Gene expression levels in SubtiExpress: cdaS | |
| Metabolic function and regulation of this protein in SubtiPathways: CdaS | |
| MW, pI | 22 kDa, 5.367 |
| Gene length, protein length | 621 bp, 207 aa |
| Immediate neighbours | yojK, yojI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19430
Phenotypes of a mutant
Database entries
- BsubCyc: BSU19430
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaA
Extended information on the protein
- Kinetic information:
- Domains:
- N-terminal autoinhibitory domain PubMed
- C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Effectors of protein activity:
- Interactions:
- forms hexamers (enzymatically inactive) or dimers (active) PubMed
- Localization:
Database entries
- BsubCyc: BSU19430
- Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
- UniProt: O31854
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP983 (cdaS::ermC), available in Jörg Stülke's lab
- GP1360 (cdaS::spc), available in Jörg Stülke's lab
- Expression vector:
- IPTG inducible expression of B. cereus Strep-cdaS in E. coli: pGP2593 (in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- His-tag fusion:
- pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
- pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
