Difference between revisions of "PpaC"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 4150 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 10163 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 10:39, 17 April 2014

  • Description: inorganic pyrophosphatase

Gene name ppaC
Synonyms yybQ
Essential yes PubMed
Product inorganic pyrophosphatase
Function recovery of phosphate ions from pyrophosphate
Gene expression levels in SubtiExpress: ppaC
MW, pI 33 kDa, 4.513
Gene length, protein length 927 bp, 309 aa
Immediate neighbours hypR, yybP
Sequences Protein DNA DNA_with_flanks
Genetic context
PpaC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

phosphate metabolism, essential genes, most abundant proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Diphosphate + H2O = 2 phosphate (according to Swiss-Prot)
  • Protein family: PPase class C family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • S-cysteinylation after diamide stress (C158) PubMed
    • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 4150 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 10163 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References