SacT

• Description: transcriptional antiterminator for the sacP-sacA-ywdA operon
  
  

Gene name	sacT
Synonyms	ipa-47d
Essential	no
Product	transcriptional antiterminator
Function	regulation of sucrose utilization
Gene expression levels in SubtiExpress: sacT
Interactions involving this protein in SubtInteract: SacT
Metabolic function and regulation of this protein in SubtiPathways: sacT
MW, pI	31 kDa, 5.587
Gene length, protein length	828 bp, 276 aa
Immediate neighbours	ywcJ, ywcI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

The gene

Basic information

• Locus tag: BSU38070

Database entries

• BsubCyc: BSU38070

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

• Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators

• Paralogous protein(s): LicT, SacY, GlcT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PtsH-SacT

• Localization:

Database entries

• BsubCyc: BSU38070

• Structure:

• UniProt: P26212

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sacT PubMed

• Expression browser: sacT PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP429 (spc), available in Stülke lab

• Expression vector:
  • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Stülke lab
  • for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Stülke lab
  • for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Stülke lab
  • for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Stülke lab
  • for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Stülke lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Stülke lab
  • for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Stülke lab
  • for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Stülke lab
  • for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Stülke lab

• lacZ fusion:

• GFP fusion: GP1227 (spc, based on pGP1870), available in the Stülke lab

• YFP fusion: GP1231 (spc, based on pGP1871), available in the Stülke lab

• FLAG-tag construct: GP1223 (spc, based on pGP1331), available in the Stülke lab

• two-hybrid system:

• Antibody:

References

Sebastian Hübner, Nathalie Declerck, Christine Diethmaier, Dominique Le Coq, Stephane Aymerich, Jörg Stülke
Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family.
Nucleic Acids Res: 2011, 39(10);4360-72
[PubMed:21278164] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

M Arnaud, M Débarbouillé, G Rapoport, M H Saier, J Reizer
In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.
J Biol Chem: 1996, 271(31);18966-72
[PubMed:8702561] [WorldCat.org] [DOI] (P p)

S Aymerich, M Steinmetz
Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family.
Proc Natl Acad Sci U S A: 1992, 89(21);10410-4
[PubMed:1279678] [WorldCat.org] [DOI] (P p)

M Arnaud, P Vary, M Zagorec, A Klier, M Debarbouille, P Postma, G Rapoport
Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity.
J Bacteriol: 1992, 174(10);3161-70
[PubMed:1577686] [WorldCat.org] [DOI] (P p)

M Debarbouille, M Arnaud, A Fouet, A Klier, G Rapoport
The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators.
J Bacteriol: 1990, 172(7);3966-73
[PubMed:2163394] [WorldCat.org] [DOI] (P p)