YwlF

• Description: ribose-5-phosphate isomerase
  
  

Gene name	ywlF
Synonyms	ipc-32d
Essential	no
Product	ribose-5-phosphate isomerase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ywlF
Metabolic function and regulation of this protein in SubtiPathways: ywlF
MW, pI	16 kDa, 5.416
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	ywlG, ywlE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36920

Database entries

• BsubCyc: BSU36920

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lacAB/rpiB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible Mass Action Kinetics PubMed PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • Inhibited by 6-phosphogluconate and AMP PubMed
  • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
  • Activated by EDTA and 2-mercaptoethanol PubMed

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU36920

• Structure: 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)

• UniProt: P39156

• KEGG entry: [3]

• E.C. number: 5.3.1.6

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

• Operon: ywlF-ywlG PubMed

• Expression browser: ywlF PubMed

• Sigma factor:

• Regulation:
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 367 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1467 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1404 (spc, based on pGP1331), available in the Stülke lab

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

R D MacElroy, C R Middaugh
Bacterial ribosephosphate isomerase.
Methods Enzymol: 1982, 89 Pt D;571-9
[PubMed:7144591] [WorldCat.org] [DOI] (P p)

C R Middaugh, R D MacElroy
The effect of temperature on ribose-5-phosphate isomerase from a mesophile, Thiobacillus thioparus, and a thermophile, Bacillus caldolyticus.
J Biochem: 1976, 79(6);1331-44
[PubMed:956158] [WorldCat.org] [DOI] (P p)