Difference between revisions of "MifM"
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqzJ_2483586_2483873_1 mifM] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqzJ_2483586_2483873_1 mifM] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed> 19779460 21383133 20525796 22456704 22383849 22864117 </pubmed> | + | <pubmed> 19779460 21383133 20525796 22456704 22383849 22864117 25313395 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:29, 19 October 2014
- Description: ribosome-nascent chain sensor of membrane protein biogenesis
| Gene name | mifM |
| Synonyms | yqzJ |
| Essential | no |
| Product | sensor of SpoIIIJ activity |
| Function | control of yidC2 translation |
| Gene expression levels in SubtiExpress: mifM | |
| Interactions involving this protein in SubtInteract: MifM | |
| MW, pI | 11 kDa, 5.829 |
| Gene length, protein length | 285 bp, 95 aa |
| Immediate neighbours | polY1, yidC2 |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, membrane proteins
This gene is a member of the following regulons
The MifM regulon: yidC2
The gene
Basic information
- Locus tag: BSU23880
Phenotypes of a mutant
Database entries
- BsubCyc: BSU23880
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- membrane PubMed
Database entries
- BsubCyc: BSU23880
- Structure:
- UniProt: Q7WY64
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for mifM PubMed
- MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
- elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Shinobu Chiba, Koreaki Ito
MifM monitors total YidC activities of Bacillus subtilis, including that of YidC2, the target of regulation.
J Bacteriol: 2015, 197(1);99-107
[PubMed:25313395]
[WorldCat.org]
[DOI]
(I p)
Shinobu Chiba, Koreaki Ito
Multisite ribosomal stalling: a unique mode of regulatory nascent chain action revealed for MifM.
Mol Cell: 2012, 47(6);863-72
[PubMed:22864117]
[WorldCat.org]
[DOI]
(I p)
Gene-Wei Li, Eugene Oh, Jonathan S Weissman
The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.
Nature: 2012, 484(7395);538-41
[PubMed:22456704]
[WorldCat.org]
[DOI]
(I e)
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
Shinobu Chiba, Takashi Kanamori, Takuya Ueda, Yoshinori Akiyama, Kit Pogliano, Koreaki Ito
Recruitment of a species-specific translational arrest module to monitor different cellular processes.
Proc Natl Acad Sci U S A: 2011, 108(15);6073-8
[PubMed:21383133]
[WorldCat.org]
[DOI]
(I p)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460]
[WorldCat.org]
[DOI]
(I p)
