MetS

• Description: methionyl-tRNA synthetase
  
  

Gene name	metS
Synonyms
Essential	yes PubMed
Product	methionyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: metS
Metabolic function and regulation of this protein in SubtiPathways: metS
MW, pI	76 kDa, 4.958
Gene length, protein length	1992 bp, 664 aa
Immediate neighbours	abrB, yabD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU00380

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met) (according to Swiss-Prot)

• Protein family: tRNA-binding domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-652 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU00380

• Structure: 1WE0 (from Thermus thermophilus hb8 mutant, 40% identity, 57% similarity) PubMed

• UniProt: P37465

• KEGG entry: [3]

• E.C. number: 6.1.1.10

Expression and regulation

• Operon: metS

• Expression browser: metS PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1310 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4911 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)