Difference between revisions of "DarA"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
**pGP2601: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
**pGP2601: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 +
 +
**pGP2603: expression of ''yaaQ''-Strep by [[pGP382]] in ''B. subtilis'' suitable for [[SPINE]], available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 09:31, 14 March 2014

  • Description: unknown

Gene name yaaQ
Synonyms
Essential
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yaaQ
MW, pI 11 kDa, 6.088
Gene length, protein length 327 bp, 109 aa
Immediate neighbours tmk, yaaR
Sequences Protein DNA DNA_with_flanks
Genetic context
YaaQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YaaQ expression.png















Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00290

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3M05 (the protein from Pediococcus pentosaceus, 50% identity)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • pGP2601: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References