SspA

• Description: small acid-soluble spore protein (major alpha-type SASP)
  
  

Gene name	sspA
Synonyms
Essential	no
Product	small acid-soluble spore protein (major alpha-type SASP)
Function	protection of spore DNA
Gene expression levels in SubtiExpress: sspA
MW, pI	6 kDa, 4.731
Gene length, protein length	207 bp, 69 aa
Immediate neighbours	ytcI, thiI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU29570

Phenotypes of a mutant

• a sspA mutant is sensitive to ionizing radiation PubMed

Database entries

• BsubCyc: BSU29570

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: alpha/beta-type SASP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29570

• Structure: 2BZ0 (82% identity, 89% similarity)

• UniProt: P04831

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sspA PubMed

• Expression browser: sspA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • BP137 (sspA::cat) available in Fabian Commichau's lab
  • 1S109 (no resistance), available at BGSC
  • 1S111 (no resistance), PubMed, available at BGSC
  • 1S112 (no resistance), PubMed, available at BGSC
  • 1S113 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: