Difference between revisions of "Nfo"

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(Original publications)
(Phenotypes of a mutant)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
** an ''[[exoA]] [[nfo]]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[[disA]]'' {{PubMed|24244006}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 12:52, 27 November 2013

  • Description: type IV apurinic/apyrimidinic endonuclease

Gene name nfo
Synonyms yqfS
Essential no
Product type IV apurinic/apyrimidinic endonuclease
Function repair of oxidative DNA damage in spores
Gene expression levels in SubtiExpress: nfo
MW, pI 32 kDa, 5.371
Gene length, protein length 891 bp, 297 aa
Immediate neighbours yqfT, cshB
Sequences Protein DNA DNA_with_flanks
Genetic context
YqfS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Nfo expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU25130

Phenotypes of a mutant

    • an exoA nfo double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of disA PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • Nfo is functionally redundant with ExoA

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)
  • Protein family: AP endonuclease 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

The gene yqfT is located between nfo and yqfU, but is transcribed in the opposite direction.

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications